Part:BBa_K103019:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

'Hunter and prey' selection system

This part was used as core of prey generator device. It was fused with:

• protein A BBa_K103003. Resulting fusion was named His_alpha_A
• protein Z_SPA-1 BBa_K103004. Resulting fusion was named His_alpha_Z

Both fusions were overexpressed in Roseta strain. Due to ability of protein Z to form aggregates His_alpha_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. His_alpha_A was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008&arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha purified] on NiNTA-agarose bead (elution peak in 150 mM imidazole). The both prey protein fusions were added to culture media and used in selection experiments. Details here and here.

User Reviews

UNIQb975ad865159332c-partinfo-00000000-QINU UNIQb975ad865159332c-partinfo-00000001-QINU